Journal: Biomolecules

Article Title: Critical Assessment of Methods to Quantify Biofilm Growth and Evaluate Antibiofilm Activity of Host Defence Peptides

doi: 10.3390/biom8020029

Figure Lengend Snippet: Effect of media composition and temperature on Pseudomonas aeruginosa biofilm growth when grown in glass tubes under static conditions. PA14 and PAO1 biofilms were grown under static conditions in glass culture tubes in modified basal medium 2 (BM2) minimal media or dilutions of rich media (tryptic soy broth (TSB) and Luria broth (LB)) at 37 °C (overnight) or 21 °C (two days). The following day, ( A ) the cultures were rinsed, stained with 0.1% crystal violet (CV), rinsed again, and imaged. The purple staining pattern represented biofilm biomass. ( B ) The CV was released by the addition of 70% ethanol and quantified by reading on a spectrometer. The samples were run in triplicate, averaged, and the error bars represent standard deviation.

Article Snippet: Other media included Luria Broth (LB, Thermo Fisher Scientific Cat# BP1427-500) and tryptic soy broth (TSB, BD Bacto, Thermo Fisher Scientific Cat# DF0370-17-3), made according to the manufacturers specifications and then evaluated by diluting in sterile water at various strengths ( v / v : 100%, 50%, 10%, 2%, or 1%) or supplementing with different concentrations of glucose (1% or 0.1%).

Techniques: Modification, Staining, Standard Deviation